Robust expansion of dendritic cells in vivo by hydrodynamic FLT3L-FC gene transfer
Due to low numbers of endogenous dendritic cells (DCs) in vivo, exogenous DC-poietin Fms-
like tyrosine kinase 3-ligand (FLT3L) is routinely used to generate DC for subsequent
studies. We engineered a novel FLT3L-FC DNA construct that, when combined with
hydrodynamic gene transfer (HDT), induced robust DC expansion in mice. DC generated in
vivo by FLT3L-FC HDT produced cytokines in response to stimulation by an array of TLR
agonists and promoted T cell proliferation. The FLT3L-FC protein produced in vivo …
like tyrosine kinase 3-ligand (FLT3L) is routinely used to generate DC for subsequent
studies. We engineered a novel FLT3L-FC DNA construct that, when combined with
hydrodynamic gene transfer (HDT), induced robust DC expansion in mice. DC generated in
vivo by FLT3L-FC HDT produced cytokines in response to stimulation by an array of TLR
agonists and promoted T cell proliferation. The FLT3L-FC protein produced in vivo …