JAK inhibitors disrupt T cell-induced proinflammatory macrophage activation
MH Nyirenda, JS Nijjar, M Frleta-Gilchrist… - RMD open, 2023 - rmdopen.bmj.com
MH Nyirenda, JS Nijjar, M Frleta-Gilchrist, DS Gilchrist, D Porter, S Siebert, CS Goodyear…
RMD open, 2023•rmdopen.bmj.comObjectives Macrophage subsets, activated by T cells, are increasingly recognised to play a
central role in rheumatoid arthritis (RA) pathogenesis. Janus kinase (JAK) inhibitors have
proven beneficial clinical effects in RA. In this study, we investigated the effect of JAK
inhibitors on the generation of cytokine-activated T (Tck) cells and the production of
cytokines and chemokines induced by Tck cell/macrophage interactions. Methods CD14+
monocytes and CD4+ T cells were purified from peripheral blood mononuclear cells from …
central role in rheumatoid arthritis (RA) pathogenesis. Janus kinase (JAK) inhibitors have
proven beneficial clinical effects in RA. In this study, we investigated the effect of JAK
inhibitors on the generation of cytokine-activated T (Tck) cells and the production of
cytokines and chemokines induced by Tck cell/macrophage interactions. Methods CD14+
monocytes and CD4+ T cells were purified from peripheral blood mononuclear cells from …
Objectives
Macrophage subsets, activated by T cells, are increasingly recognised to play a central role in rheumatoid arthritis (RA) pathogenesis. Janus kinase (JAK) inhibitors have proven beneficial clinical effects in RA. In this study, we investigated the effect of JAK inhibitors on the generation of cytokine-activated T (Tck) cells and the production of cytokines and chemokines induced by Tck cell/macrophage interactions.
Methods
CD14+ monocytes and CD4+ T cells were purified from peripheral blood mononuclear cells from buffy coats of healthy donors. As representative JAK inhibitors, tofacitinib or ruxolitinib were added during Tck cell differentiation. Previously validated protocols were used to generate macrophages and Tck cells from monocytes and CD4+ T cells, respectively. Cytokine and chemokine including TNF, IL-6, IL-15, IL-RA, IL-10, MIP1α, MIP1β and IP10 were measured by ELISA.
Results
JAK inhibitors prevented cytokine-induced maturation of Tck cells and decreased the production of proinflammatory cytokines TNF, IL-6, IL-15, IL-1RA and the chemokines IL-10, MIP1α, MIP1β, IP10 by Tck cell-activated macrophages in vitro (p<0.05).
Conclusions
Our findings show that JAK inhibition disrupts T cell-induced macrophage activation and reduces downstream proinflammatory cytokine and chemokine responses, suggesting that suppressing the T cell-macrophage interaction contributes to the therapeutic effect of JAK inhibitors.
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