[HTML][HTML] Rho kinase inhibition rescues the endothelial cell cerebral cavernous malformation phenotype
AL Borikova, CF Dibble, N Sciaky, CM Welch… - Journal of biological …, 2010 - ASBMB
Cerebral cavernous malformations (CCM) are vascular lesions causing seizures and stroke.
Mutations causing inactivation of one of three genes, ccm1,-2, or-3, are sufficient to induce
vascular endothelial cell defects resulting in CCM. Herein, we show that loss of expression
of the CCM1,-2, or-3 proteins causes a marked increase in expression of the GTPase RhoA.
Live cell imaging with a RhoA-specific biosensor demonstrates increased RhoA activity with
loss of CCM1,-2, or-3, with an especially pronounced RhoA activation in both the cytosol …
Mutations causing inactivation of one of three genes, ccm1,-2, or-3, are sufficient to induce
vascular endothelial cell defects resulting in CCM. Herein, we show that loss of expression
of the CCM1,-2, or-3 proteins causes a marked increase in expression of the GTPase RhoA.
Live cell imaging with a RhoA-specific biosensor demonstrates increased RhoA activity with
loss of CCM1,-2, or-3, with an especially pronounced RhoA activation in both the cytosol …