New aspects of siglec binding specificities, including the significance of fucosylation and of the sialyl-Tn epitope
ECM Brinkman-Van der Linden, A Varki - Journal of Biological Chemistry, 2000 - ASBMB
The siglecs (sialic acid-bindingimmunoglobulin superfamilylectins) are immunoglobulin
superfamily members recognizing sialylated ligands. Most prior studies of siglec specificities
focused on α2–3-and α2–6-sialyllactos (amin) es and on one or two of the siglecs at a time.
Here, we explore several new aspects of specificities of the first six reported siglecs, using
sialylated glycans presented in multivalent form, on synthetic polyacrylamide backbones, or
on mucin polypeptides. First, we report that binding of siglec-1 (sialoadhesin), siglec-3 …
superfamily members recognizing sialylated ligands. Most prior studies of siglec specificities
focused on α2–3-and α2–6-sialyllactos (amin) es and on one or two of the siglecs at a time.
Here, we explore several new aspects of specificities of the first six reported siglecs, using
sialylated glycans presented in multivalent form, on synthetic polyacrylamide backbones, or
on mucin polypeptides. First, we report that binding of siglec-1 (sialoadhesin), siglec-3 …
