Relocalization of junctional adhesion molecule A during inflammatory stimulation of brain endothelial cells

SM Stamatovic, N Sladojevic, RF Keep… - … and cellular biology, 2012 - Taylor & Francis
SM Stamatovic, N Sladojevic, RF Keep, AV Andjelkovic
Molecular and cellular biology, 2012Taylor & Francis
Junctional adhesion molecule A (JAM-A) is a unique tight junction (TJ) transmembrane
protein that under basal conditions maintains endothelial cell-cell interactions but under
inflammatory conditions acts as a leukocyte adhesion molecule. This study investigates the
fate of JAM-A during inflammatory TJ complex remodeling and paracellular route formation
in brain endothelial cells. The chemokine (CC motif) ligand 2 (CCL2) induced JAM-A
redistribution from the interendothelial cell area to the apical surface, where JAM-A played a …
Junctional adhesion molecule A (JAM-A) is a unique tight junction (TJ) transmembrane protein that under basal conditions maintains endothelial cell-cell interactions but under inflammatory conditions acts as a leukocyte adhesion molecule. This study investigates the fate of JAM-A during inflammatory TJ complex remodeling and paracellular route formation in brain endothelial cells. The chemokine (C-C motif) ligand 2 (CCL2) induced JAM-A redistribution from the interendothelial cell area to the apical surface, where JAM-A played a role as a leukocyte adhesion molecule participating in transendothelial cell migration of neutrophils and monocytes. JAM-A redistribution was associated with internalization via macropinocytosis during paracellular route opening. A tracer study with dextran-Texas Red indicated that internalization occurred within a short time period (∼10 min) by dextran-positive vesicles and then became sorted to dextran-positive/Rab34-positive/Rab5-positive vesicles and then Rab4-positive endosomes. By ∼20 min, most internalized JAM-A moved to the brain endothelial cell apical membrane. Treatment with a macropinocytosis inhibitor, 5-(N-ethyl-N-isopropyl)amiloride, or Rab5/Rab4 depletion with small interfering RNA oligonucleotides prevented JAM-A relocalization, suggesting that macropinocytosis and recycling to the membrane surface occur during JAM-A redistribution. Analysis of the signaling pathways indicated involvement of RhoA and Rho kinase in JAM-A relocalization. These data provide new insights into the molecular and cellular mechanisms involved in blood-brain barrier remodeling during inflammation.
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