Surfactant stimulates prostaglandin E production in human amnion
AL Bernal, GE Newman… - … Journal of Obstetrics …, 1988 - Wiley Online Library
AL Bernal, GE Newman, PJR Phizackerley, AC Turnbull
BJOG: An International Journal of Obstetrics & Gynaecology, 1988•Wiley Online LibraryDiscs of human amnion prepared from nine women delivered by elective caesarean section
at term were incubated with and without purified human amniotic fluid surfactant (9 μg lipid
P/ml), and the output of prostaglandin E (PGE) was estimated by radioimmunoassay.
Surfactant stimulated the release of PGE from 3· 8 (SD 2· 9) to 5· 4 (SD 2· 5) pmol/mg dry
weight/3 h (P< 0· 01). Arachidonic acid accounted for 2% of the fatty acids of surfactant
lecithin. These results show that surfactant has a stimulatory effect on amniotic PGE …
at term were incubated with and without purified human amniotic fluid surfactant (9 μg lipid
P/ml), and the output of prostaglandin E (PGE) was estimated by radioimmunoassay.
Surfactant stimulated the release of PGE from 3· 8 (SD 2· 9) to 5· 4 (SD 2· 5) pmol/mg dry
weight/3 h (P< 0· 01). Arachidonic acid accounted for 2% of the fatty acids of surfactant
lecithin. These results show that surfactant has a stimulatory effect on amniotic PGE …
Summary
Discs of human amnion prepared from nine women delivered by elective caesarean section at term were incubated with and without purified human amniotic fluid surfactant (9 μg lipid P/ml), and the output of prostaglandin E (PGE) was estimated by radioimmunoassay. Surfactant stimulated the release of PGE from 3·8 (SD 2·9) to 5·4 (SD 2·5) pmol/mg dry weight/3 h (P<0·01). Arachidonic acid accounted for 2% of the fatty acids of surfactant lecithin. These results show that surfactant has a stimulatory effect on amniotic PGE production and that it is an important source of arachidonic acid in amniotic fluid.
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