RNA is more UV resistant than DNA: The formation of UV‐induced DNA lesions is strongly sequence and conformation dependent
LM Kundu, U Linne, M Marahiel… - Chemistry–A European …, 2004 - Wiley Online Library
Chemistry–A European Journal, 2004•Wiley Online Library
DNA and RNA hairpins, which represent well‐folded oligonucleotide structures, were
irradiated and the amount of damaged hairpins was directly quantified by using ion‐
exchange HPLC. The types of photoproducts formed in the hairpins were determined by ESI‐
HPLC‐MS/MS experiments. Irradiation of hairpins with systematically varied sequences and
conformations (A versus B) revealed remarkable differences regarding the amount of
photolesions formed. UV‐damage formation is, therefore, a strongly sequence and …
irradiated and the amount of damaged hairpins was directly quantified by using ion‐
exchange HPLC. The types of photoproducts formed in the hairpins were determined by ESI‐
HPLC‐MS/MS experiments. Irradiation of hairpins with systematically varied sequences and
conformations (A versus B) revealed remarkable differences regarding the amount of
photolesions formed. UV‐damage formation is, therefore, a strongly sequence and …
Abstract
DNA and RNA hairpins, which represent well‐folded oligonucleotide structures, were irradiated and the amount of damaged hairpins was directly quantified by using ion‐exchange HPLC. The types of photoproducts formed in the hairpins were determined by ESI‐HPLC‐MS/MS experiments. Irradiation of hairpins with systematically varied sequences and conformations (A versus B) revealed remarkable differences regarding the amount of photolesions formed. UV‐damage formation is, therefore, a strongly sequence and conformation dependent process.
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