Functional transplantation of the sumoylation machinery into Escherichia coli

M Mencía, V de Lorenzo - Protein expression and purification, 2004 - Elsevier
Modification by SUMO proteins appears to be very common in eukaryotic cells. Many
proteins have been reported to be sumoylated, at least under certain circumstances, in vivo,
and new examples get published every month. On the other hand, sumoylation is, in
essence, a way to construct branched proteins or protein fusions. Obtention of pure
sumoylated proteins from eukaryotic cells is not easy because of the dynamic nature of this
modification and the large number of sumoylated proteins in vivo. Production of sumoylated …