Mutational analysis of critical residues determining antigen presentation and activation of HLA-DQ0602 restricted T-cell clones
S Reichstetter, GK Papadopoulos, AK Moustakas… - Human immunology, 2002 - Elsevier
Three different HLA-DQ0602 restricted T-lymphocyte clones (clones 5, 44, and 48) specific
for two different Herpes simplex virus type 2 (HSV-2) VP16 peptides were used in a series of
proliferation assays with BLS-1 cell lines expressing mutated HLA-DQ0604 molecules as
APC. Up to four residues in the peptide-binding region of DQ0604 were replaced by the
respective DQ0602 residue. For all three clones, residue β70 played a crucial role in TCR
recognition; β30 and β57 were important, although β86 was less significant. Clone 5 and 48 …
for two different Herpes simplex virus type 2 (HSV-2) VP16 peptides were used in a series of
proliferation assays with BLS-1 cell lines expressing mutated HLA-DQ0604 molecules as
APC. Up to four residues in the peptide-binding region of DQ0604 were replaced by the
respective DQ0602 residue. For all three clones, residue β70 played a crucial role in TCR
recognition; β30 and β57 were important, although β86 was less significant. Clone 5 and 48 …