Studies on the pathogenesis of parainfluenza type 3 virus infection in hamsters
C Liu, E Sharp, J Collins - Archiv für die gesamte Virusforschung, 1968 - Springer
C Liu, E Sharp, J Collins
Archiv für die gesamte Virusforschung, 1968•SpringerAfter intranasal inoculation of parainfluenza type 3 virus in hamsters, viral multiplication was
detectable by infectivity titrations and immunofluorescent (FA) staining to have occurred in
respiratory tissues as early as 24 hours following inoculation. Virus persisted in the
respiratory epithelium up to the 6th day of infection. By FA staining, localization of viral
antigen was seen in the cytoplasm of infected ciliated epithelial cells in the form of brilliant
fluorescent granules of varying size. Administration of corticosteroids to hamsters prolonged …
detectable by infectivity titrations and immunofluorescent (FA) staining to have occurred in
respiratory tissues as early as 24 hours following inoculation. Virus persisted in the
respiratory epithelium up to the 6th day of infection. By FA staining, localization of viral
antigen was seen in the cytoplasm of infected ciliated epithelial cells in the form of brilliant
fluorescent granules of varying size. Administration of corticosteroids to hamsters prolonged …
Summary
After intranasal inoculation of parainfluenza type 3 virus in hamsters, viral multiplication was detectable by infectivity titrations and immunofluorescent (FA) staining to have occurred in respiratory tissues as early as 24 hours following inoculation. Virus persisted in the respiratory epithelium up to the 6th day of infection. By FA staining, localization of viral antigen was seen in the cytoplasm of infected ciliated epithelial cells in the form of brilliant fluorescent granules of varying size.
Administration of corticosteroids to hamsters prolonged the parainfluenza infection to 9 to 11 days after virus inoculation. However, the amount of infectious virus and antigenic distribution showed no significant difference between treated and untreated animals.
The direct PA staining was found to be superior to the indirect method in demonstrating the antigenic localization. The reaction was specific and no cross reaction was encountered with other myxoviruses tested. Hyperimmune animal serum with high antibody titers against parainfluenza 3 virus was required to prepare satisfactory fluorescent conjugates.
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