Full length and discrete partial reverse transcripts of globin and chorion mRNAs
A Efstratiadis, T Maniatis, FC Kafatos, A Jeffrey… - Cell, 1975 - cell.com
A Efstratiadis, T Maniatis, FC Kafatos, A Jeffrey, JN Vournakis
Cell, 1975•cell.comRabbit globin mRNA was copied by AMV reverse transcriptase in the presence of various
concentrations of deoxyribonucleotides(dNTPs). The cDNAs were analyzed by
electrophoresls under denaturing conditions in formamide-polyacrylamide gels. Discrete
size products were detected, ranging from 65 to 650 nucleotides--that is, up to the full length
of the mRNA template. Increasing the concentrations of all four dNTPs stimulated formation
of fulllength transcripts and made the incomplete copies less abundant. Hybridization and …
concentrations of deoxyribonucleotides(dNTPs). The cDNAs were analyzed by
electrophoresls under denaturing conditions in formamide-polyacrylamide gels. Discrete
size products were detected, ranging from 65 to 650 nucleotides--that is, up to the full length
of the mRNA template. Increasing the concentrations of all four dNTPs stimulated formation
of fulllength transcripts and made the incomplete copies less abundant. Hybridization and …
Summary
Rabbit globin mRNA was copied by AMV reverse transcriptase in the presence of various concentrations of deoxyribonucleotides(dNTPs). The cDNAs were analyzed by electrophoresls under denaturing conditions in formamide-polyacrylamide gels. Discrete size products were detected, ranging from 65 to 650 nucleotides--that is, up to the full length of the mRNA template. Increasing the concentrations of all four dNTPs stimulated formation of fulllength transcripts and made the incomplete copies less abundant. Hybridization and nuclease digestion experiments indicated that the full-size product is indeed a complete transcript of globin mRNA. Similar results were obtained with chorion mRNAs. The possible usefulness of the discrete partial transcripts is discussed.
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