EMAP II is an endothelial cell and monocyte activating proinflammatory cytokine, which has
been demonstrated to induce endothelial cell apoptosis. In order to analyze its role in
disease models linked to inflammation and endothelial cell death, we aimed to develop a
neutralizing antibody against mouse EMAP II. Therefore, we generated rat monoclonal anti-
mouse EMAP II antibodies by immunization with recombinant full length, mouse pro-EMAP II
protein. We could identify by ELISA, hybridoma clones from fusion with mouse myeloma …