Evidence for the presence of glucose cycling in pancreatic islets of the ob/ob mouse
A Khan, V Chandramouli, CG Östenson, B Ahren… - Journal of Biological …, 1989 - ASBMB
Pancreatic islets from ob/ob mice incubated with 3 H 2 O and 5.5 mM glucose formed 3 H-
labeled glucose, 74 picoatoms incorporated/islet/h. Sixty-three percent of the 3 H was bound
to carbon 2 of the glucose. The amount of glucose-6-P dephosphorylated to glucose,
determined from this incorporation, was 48 pmol/islet/h. Glucose utilization, measured by the
formation of 3 H 2 O from [5-3 H] glucose, was 72 pmol/islet/h. The amount of glucose
dephosphorylated was then about 40% of that phosphorylated. Thus, glucose-6-P is …
labeled glucose, 74 picoatoms incorporated/islet/h. Sixty-three percent of the 3 H was bound
to carbon 2 of the glucose. The amount of glucose-6-P dephosphorylated to glucose,
determined from this incorporation, was 48 pmol/islet/h. Glucose utilization, measured by the
formation of 3 H 2 O from [5-3 H] glucose, was 72 pmol/islet/h. The amount of glucose
dephosphorylated was then about 40% of that phosphorylated. Thus, glucose-6-P is …
