Fluorimetric DNA assay for cell growth estimation
J Rao, WR Otto - Analytical biochemistry, 1992 - Elsevier
J Rao, WR Otto
Analytical biochemistry, 1992•ElsevierA growth assay using the fluorescent dye Hoechst 33258 has been developed which allows
sensitive and rapid analysis of the DNA content of a variety of cell types including
keratinocytes and mucus-secreting cells, and which requires a minimum of liquid handling.
The assay can detect as few as 500 diploid human cells, and is compatible with the
simultaneous detection of [3H] thymidine incorporation in the same cultures.
sensitive and rapid analysis of the DNA content of a variety of cell types including
keratinocytes and mucus-secreting cells, and which requires a minimum of liquid handling.
The assay can detect as few as 500 diploid human cells, and is compatible with the
simultaneous detection of [3H] thymidine incorporation in the same cultures.
A growth assay using the fluorescent dye Hoechst 33258 has been developed which allows sensitive and rapid analysis of the DNA content of a variety of cell types including keratinocytes and mucus-secreting cells, and which requires a minimum of liquid handling. The assay can detect as few as 500 diploid human cells, and is compatible with the simultaneous detection of [3H]thymidine incorporation in the same cultures.
Elsevier
