Molecular tools for genetic dissection of the protozoan parasite Toxoplasma gondii

DS Roos, RGK Donald, NS Morrissette… - Methods in cell biology, 1995 - Elsevier
Publisher Summary The genetic structure of Toxoplasma gondii is notable chiefly for being
relatively conventional—similar to that of its mammalian host cells with respect to gene
organization, codon usage, and nucleotide bias. These observations have led several
investigators to examine the feasibility of molecular transformation in this parasite. This
chapter outlines the use of several of the molecular genetic tools that have recently been
developed for the T. gondii system. An introduction to parasite culture techniques is also …