[HTML][HTML] ApoA-I secretion from HepG2 cells: evidence for the secretion of both lipid-poor apoA-I and intracellularly assembled nascent HDL
JW Chisholm, ER Burleson, GS Shelness… - Journal of lipid …, 2002 - ASBMB
The goal of this study was to determine whether apolipoprotein AI (apoA-I) is lipidated
before secretion by HepG2 cells. ApoA-I was extracted from microsomes after radiolabeling
with [35 S] Met/Cys. After ultracentrifugal flotation, d< 1.25 g/ml and d> 1.25 g/ml fractions
were immunoprecipitated and analyzed by SDS-PAGE. Under steady state radiolabeling
conditions, 20% of extracted microsomal apoA-I floated at d< 1.25 g/ml. Pulse-chase
experiments demonstrated that the percentage of microsomal apoA-I associated with lipid …
before secretion by HepG2 cells. ApoA-I was extracted from microsomes after radiolabeling
with [35 S] Met/Cys. After ultracentrifugal flotation, d< 1.25 g/ml and d> 1.25 g/ml fractions
were immunoprecipitated and analyzed by SDS-PAGE. Under steady state radiolabeling
conditions, 20% of extracted microsomal apoA-I floated at d< 1.25 g/ml. Pulse-chase
experiments demonstrated that the percentage of microsomal apoA-I associated with lipid …
