[HTML][HTML] Regulation of osteocalcin gene expression by a novel Ku antigen transcription factor complex
DM Willis, AP Loewy, N Charlton-Kachigian… - Journal of Biological …, 2002 - ASBMB
We previously described anosteocalcin (OC) fibroblast growth factor (FGF) response
element (FRE) DNA binding activity as a target of Msx2 transcriptional regulation. We now
identify Ku70, Ku80, and Tbdn100, a variant of Tubedown-1, as constituents of the purified
OCFRE-binding complex. Northern and Western blot analyses demonstrate expression of
Ku and Tbdn100 in MC3T3E1 osteoblasts. FGF2 treatment regulates Ku, but not Tbdn100,
protein accumulation. Gel supershift studies confirm sequence-specific DNA binding of Ku in …
element (FRE) DNA binding activity as a target of Msx2 transcriptional regulation. We now
identify Ku70, Ku80, and Tbdn100, a variant of Tubedown-1, as constituents of the purified
OCFRE-binding complex. Northern and Western blot analyses demonstrate expression of
Ku and Tbdn100 in MC3T3E1 osteoblasts. FGF2 treatment regulates Ku, but not Tbdn100,
protein accumulation. Gel supershift studies confirm sequence-specific DNA binding of Ku in …