[HTML][HTML] The renaturable 69-and 63-kDa protein kinases that undergo rapid activation in chemoattractant-stimulated guinea pig neutrophils are p21-activated kinases
J Ding, UG Knaus, JP Lian, GM Bokoch… - Journal of Biological …, 1996 - ASBMB
Neutrophils stimulated with the chemoattractant fMet-Leu-Phe (fMLP) are known to exhibit
rapid activation of four protein kinases with molecular masses of~ 69,~ 63,~ 49, and~ 40-
kDa. Activation of these kinases is blocked by antagonists of phosphatidylinositol 3-kinase
and type 1 and/or type 2A protein phosphatases. These enzymes can be detected by their
ability to undergo renaturation and catalyze the phosphorylation of a peptide substrate that
corresponds to amino acid residues 297-331 of the 47-kDa subunit of the NADPH-oxidase …
rapid activation of four protein kinases with molecular masses of~ 69,~ 63,~ 49, and~ 40-
kDa. Activation of these kinases is blocked by antagonists of phosphatidylinositol 3-kinase
and type 1 and/or type 2A protein phosphatases. These enzymes can be detected by their
ability to undergo renaturation and catalyze the phosphorylation of a peptide substrate that
corresponds to amino acid residues 297-331 of the 47-kDa subunit of the NADPH-oxidase …