[HTML][HTML] Characterization of rac and cdc42 activation in chemoattractant-stimulated human neutrophils using a novel assay for active GTPases
V Benard, BP Bohl, GM Bokoch - Journal of Biological Chemistry, 1999 - ASBMB
A major function of Rac2 in neutrophils is the regulation of oxidant production important in
bacterial killing. Rac and the related GTPase Cdc42 also regulate the dynamics of the actin
cytoskeleton, necessary for leukocyte chemotaxis and phagocytosis of microorganisms.
Although these GTPases appear to be critical downstream components of chemoattractant
receptor signaling in human neutrophils, the pathways involved in direct control of
Rac/Cdc42 activation remain to be determined. We describe an assay that measures the …
bacterial killing. Rac and the related GTPase Cdc42 also regulate the dynamics of the actin
cytoskeleton, necessary for leukocyte chemotaxis and phagocytosis of microorganisms.
Although these GTPases appear to be critical downstream components of chemoattractant
receptor signaling in human neutrophils, the pathways involved in direct control of
Rac/Cdc42 activation remain to be determined. We describe an assay that measures the …