Localization of vitronectin binding domain in plasminogen activator inhibitor-1.
DA Lawrence, MB Berkenpas, S Palaniappan… - Journal of Biological …, 1994 - ASBMB
Plasminogen activator inhibitor type 1 (PAI-1) is the rapid physiologic inhibitor of tissue-type
plasminogen activator and urokinase-type plasminogen activator (uPA). In plasma and the
extracellular matrix, PAI-1 is associated with the adhesive glycoprotein vitronectin. In order
to characterize the PAI-1 structural domain responsible for binding to vitronectin, the
segment of the PAI-1 cDNA encoding amino acids 13-147 (nucleotides 248-650) was
randomly mutagenized and subcloned into a bacterial expression vector containing the …
plasminogen activator and urokinase-type plasminogen activator (uPA). In plasma and the
extracellular matrix, PAI-1 is associated with the adhesive glycoprotein vitronectin. In order
to characterize the PAI-1 structural domain responsible for binding to vitronectin, the
segment of the PAI-1 cDNA encoding amino acids 13-147 (nucleotides 248-650) was
randomly mutagenized and subcloned into a bacterial expression vector containing the …