[HTML][HTML] Caspase-9 can be activated without proteolytic processing
HR Stennicke, QL Deveraux, EW Humke… - Journal of Biological …, 1999 - ASBMB
The recombinant form of the proapoptotic caspase-9 purified following expression in
Escherichia coliis processed at Asp 315, but largely inactive; however, when added to
cytosolic extracts of human 293 cells it is activated 2000-fold in the presence of cytochrome
c and dATP. Thus, the characteristic activities of caspase-9 are context-dependent, and its
activation may not recapitulate conventional caspase activation mechanisms. To explore this
hypothesis we produced recombinant forms of procaspase-9 containing mutations that …
Escherichia coliis processed at Asp 315, but largely inactive; however, when added to
cytosolic extracts of human 293 cells it is activated 2000-fold in the presence of cytochrome
c and dATP. Thus, the characteristic activities of caspase-9 are context-dependent, and its
activation may not recapitulate conventional caspase activation mechanisms. To explore this
hypothesis we produced recombinant forms of procaspase-9 containing mutations that …
