Two-dimensional electrophoresis of plasma lipoproteins: recognition of new apo AI-containing subpopulations
BF Asztalos, CH Sloop, L Wong, PS Roheim - Biochimica et Biophysica …, 1993 - Elsevier
Two-dimensional electrophoresis has been used to resolve 12 distinct apo AI-containing
high-density lipoprotein (HDL) subpopulations in human plasma. The subpopulations were
quantitated by 125 I-labeled, monospecific antibody and phosphorimaging. Modification and
standardization of the agarose electrophoresis (first dimension) enabled us to recognize
new HDL subpopulations. Lipoprotein mobilities in agarose were expressed relative to the
mobility of the sample's endogenous albumin. We demonstrated the presence of …
high-density lipoprotein (HDL) subpopulations in human plasma. The subpopulations were
quantitated by 125 I-labeled, monospecific antibody and phosphorimaging. Modification and
standardization of the agarose electrophoresis (first dimension) enabled us to recognize
new HDL subpopulations. Lipoprotein mobilities in agarose were expressed relative to the
mobility of the sample's endogenous albumin. We demonstrated the presence of …
