Overexpression of human lecithin cholesterol acyltransferase leads to hyperalphalipoproteinemia in transgenic mice
BL Vaisman, HG Klein, M Rouis, AM Bérard… - Journal of Biological …, 1995 - ASBMB
Lecithin cholesterol acyltransferase (LCAT) is a key enzyme which catalyzes the
esterification of free cholesterol present in plasma lipoproteins. In order to evaluate the role
of LCAT in HDL metabolism, a 6.2-kilobase (kb) fragment consisting of 0.851 and 1.134 kb
of the 5'-and 3'-flanking regions, as well as the entire human LCAT gene, was utilized to
develop transgenic mice. Three different transgenic mouse lines overexpressing human
LCAT at plasma levels 11-, 14-, and 109-fold higher than non-transgenic mice were …
esterification of free cholesterol present in plasma lipoproteins. In order to evaluate the role
of LCAT in HDL metabolism, a 6.2-kilobase (kb) fragment consisting of 0.851 and 1.134 kb
of the 5'-and 3'-flanking regions, as well as the entire human LCAT gene, was utilized to
develop transgenic mice. Three different transgenic mouse lines overexpressing human
LCAT at plasma levels 11-, 14-, and 109-fold higher than non-transgenic mice were …
